Monoclonal Anti-CD3 antibody produced in mouse, clone OKT3, purified immunoglobulin, buffered aqueous solution

Application

Monoclonal Anti-CD3 antibody produced in mouse has been used in proliferation assay.

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1 µg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

T-cell receptor-CD3 complex including CD3ε, CD3γ and CD3δ, plays a vital role in inducing early metabolic events that lead to T cell activation. Mutation in the CD3ε has been observed in T-B⁺ NK⁺ severe combined immunodeficiency (SCID) patients.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

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General description

The mouse monoclonal antibody OKT3 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

The CD3 gene is mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.